Morphological heterogeneity of HeLa cell mitochondria visualized by a modified diaminobenzidine staining technique

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Morphological heterogeneity of HeLa cell mitochondria visualized by a modified diaminobenzidine staining technique.

The diaminobenzidine (DAB) technique for the ultrastructural localization of sites of cytochrome c oxidase activity in animal tissues has been adapted to the visualization of mitochondria in animal cells growing in culture. The modified technique allows the staining of mitochondria in all cells in coverslip preparatins for light microscopy. Electron microscopy of thin sections of material treat...

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Unambiguous identification of lymphocytes is sometimes difficult because of weak immunostaining of the cell membrane immunoglobulins. A simple method of intensifying the diaminobenzidine (DAB) peroxidase reaction was therefore devised. Paraffin wax sections of formalin fixed tonsils and lymphomas were digested with trypsin and immunostained for K and A light immunoglobulin chains and CD3 antige...

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ژورنال

عنوان ژورنال: Journal of Cell Science

سال: 1975

ISSN: 1477-9137,0021-9533

DOI: 10.1242/jcs.19.2.315